Rabbits were immunized twice with monovalent stock solution inactivate vaccine made from the supernatant of different generations of the two strains of virus. The neutralizing titers of the immune serum to the same strain reached l:10. OBJECTIVE: To investigate whether mouse pox exists in the experimental mice by the virus separation and the characteristics study on molecular biology. Fermentation broth was used in research experiments for antagonizing pathogenic fungi on fruits and vegetables such as green pepper Phytophthora pathogen Phytophthora capsici, pathogen of tomato Botrytis cinerea, pathogen Fusarium oxysporum f.sp. cucumerinum, cucumber Botrytis cinerea Pers, apple Venturia inaeualis, cabbage Ahernaria brassicae. The results showed that the broth of 2 bacteria strains had significantly inhibitory effects to green pepper Phytophthora pathogen, 1 strain had better inhibitory effects to pathogen of Fusarium oxysporum f.sp. cucumerinum, and 1 strain had certain inhibitory effects to pathogen of tomato Botrytis cinerea. soluble rSEA could be purified through Ni2+ affinity chromatography, and reached electrophoresis purity. Results: RT-PCR products, pGEM-T-Sj14-3-3 and pBK-Sj14-3-3 were digested separately by double-enzyme digestion and all obtained one specific gene fragment. Conclusion: In this study, it is the first time that drug-resistant S. typhi plasmid pRST98 is proved to be pleiotropic at the nucleic acid level. It is a mosaic-like plasmid carrying genes encoding not only drug resistance but also bacterial virulence. Objective: to study changes of Shiga toxin types of E. coli O157:H7 strain isolated from China. A co-transfection experiment was carried out to the eukaryotic IBRS-2 cells with the pUSK-NS1 and the genome of TK~-/gG~-/LacZ~+ mutant of the Ea strain of pseudo-rabies virus. The recombinant virus TK~-/gG~-/NS1~+ was obtained by plaque purifications. The results demonstrated that the cis-acting elements regulating the transcription activity still existed in the ...312 1080 region with the missing Xylanase II promoter. At the end of the 12th week, gastric half emptying time was measured to assess the effect of BTA on gastric motility followed by measuring the acetylcholinesterase (AchE)expression in antrum by immunohistochemistry.